TruSpectral Technology
High Sensitivity Multi-Color Imaging
Bright Multicolor Fluorescence
Integrated in FV3000 confocal systems, TruSpectral technology enables much higher light throughput compared to conventional spectral detection units.
- Volume phase holograms diffract light more efficiently than traditional reflection gratings, delivering up to three-fold higher transmission for red, far-red and near infra-red wavelength
- Bright multicolor fluorescence with low excitation light for live and fixed tissue imaging
Mouse mPFC labeled with glial fibrillary acidic protein (GFAP; astrocyte marker; yellow), calmodulin-dependent protein kinase II (CaMKII; pyramidal neuron marker; red), amphoterin-induced protein 1 precursor (AMIGO-1; neuronal membrane marker; cyan), parvalbumin (PV; inhibitory neuron marker; purple), ankyrin-G (AnkG; axon initial segment marker; green), and nuclear yellow (nuclei marker; blue).
Image data courtesy of Stephanie Shiers, Ph.D. Candidate, Theodore J. Price, Ph.D., Price Lab, Center for Advanced Pain Studies, Department of Neurobiology, School of Behavioral and Brain Sciences, University of Texas at Dallas.
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Sensitivity and Accuracy
TruSpectral detectors combine high sensitivity with spectral flexibility to detect even the dimmest fluorophores.
- Independently adjustable channels to optimize signal detection for each fluorophore
- Lambda scanning mode enables accurate spectral unmixing of complex overlapping fluorescent signals
- Variable barrier filter mode provides simultaneous four-channel image acquisition, with up to sixteen channels in virtual channel mode
- Adjustable slit to select the detection wavelengths of each individual channel down to 2 nm
- Optional Cooled Gallium Arsenide Phosphide (GaAsP) PMT detector to maximize detection efficiency
Learn More
Multiplexing with the FLUOVIEW FV3000 Confocal Microscope
Multiplexing with the FLUOVIEW FV3000 Confocal Microscope
https://main--eds-evident-website--evident-scientific.hlx.live/en/applications/spheroid_3d_imaging/
3D Time-Lapse Imaging of Spheroids with the FLUOVIEW FV3000 Confocal Microscope
3D Time-Lapse Imaging of Spheroids with the FLUOVIEW FV3000 Confocal Microscope
https://main--eds-evident-website--evident-scientific.hlx.live/en/applications/cleared-mouse-liver/
3D Observation of Cleared Mouse Liver Using the FLUOVIEW FV3000 Microscope
3D Observation of Cleared Mouse Liver Using the FLUOVIEW FV3000 Microscope
https://main--eds-evident-website--evident-scientific.hlx.live/en/applications/fucci_ca_fv3000/
Development of a New Fucci(CA) Application: A Fluorescent Probe for Visualizing Cell Cycles
Development of a New Fucci(CA) Application: A Fluorescent Probe for Visualizing Cell Cycles
Related Products
FV3000
- Available in galvanometer-only (FV3000) or hybrid galvanometer/resonant (FV3000RS) scanner configurations
- Highly efficient and accurate TruSpectral detection on all channels
- Optimized for live cell imaging with high sensitivity and low phototoxicity
Seamless deconvolution for clearer, sharper images.
Stable focus throughout time-lapse experiments.
2Automated correction for brighter and sharper images at depth.
Accurate and efficient image analysis based on deep-learning technology.